‘Protected DNA Probes’ capable of strong hybridization without removal of base protecting groups

‘Protected DNA Probes’ capable of strong hybridization without removal of base protecting groups

We propose a new strategy called the 'Protected DNA Probes (PDP) method' in which appropriately protected bases selectively bind to the complementary bases without the removal of their base protecting groups. Previously, we reported that 4-N-acetylcytosine oligonucleotides (ac(4)C) exhibited a higher hybridization affinity for ssDNA than the unmodified oligonucleotides. For the PDP strategy, we...

a generalization of strong causality

در این رساله t_n - علیت قوی تعریف می شود. این رده ها در جدول علیت فضا- زمان بین علیت پایدار و علیت قوی قرار دارند. یک قضیه برای رده بندی آنها ثابت می شود و t_n- علیت قوی با رده های علی کارتر مقایسه می شود. همچنین ثابت می شود که علیت فشرده پایدار از t_n - علیت قوی نتیجه می شود. بعلاوه به بررسی رابطه نظریه دامنه ها با نسبیت عام می پردازیم و ثابت می کنیم که نوع خاصی از فضا- زمان های علی پایدار, ب...

Labeling of in situ hybridization DNA probes

Protecting Groups, Acid, Base, Temperature and Time

Factors affecting aspartimide formation, such as protecting groups, acidity, basicity, and temperature, were studied using the model tetrapeptide, Glu-AspGly-Thr. The aspartyl carboxyl side chain in this tetrapeptide was either free or protected as a benzyl or cyclohexyl ester. Our results showed that the cyclohexyl ester led to far less aspartimide formation during acidic or tertiary amine tre...

Kinetics of base stacking-aided DNA hybridization.

The association and dissociation rate constants (k(a) and k(d)) of DNA hybridizations involving dual, single or no stacking with different base-pairing sizes were measured, which reveals the advantage of stacking hybridization in both the kinetic and steady state.